Know a Test - ActiveLAB: Active Wellness
Moderator Request
Test of the Day: Bilirubin, total


Biochemistry and Urinalysis

Test Type

Individual Test


Bile, Conjugated and unconjugated bilirubin, TBIL, T bili


Bilirubin is a bile pigment formed by the breakdown of hemoglobin during both normal and abnormal erythrocyte destruction. Direct (conjugated) bilirubin is that portion of the bilirubin that has been taken up by the liver cells to form bilirubin diglucuronide. Indirect (unconjugated) bilirubin is that portion of the bilirubin that has not been taken up by the liver cells. Total bilirubin is the sum of direct and indirect bilirubin present in the specimen. Method is diazotization. Spectrophotometry may be used in neonates six weeks or younger.

Prerequisites for tests

No special preparation is needed.


Specimen Type

1 0.2 mL serum

Sample Management

1 Collect specimen in an SST tube. Centrifuge; aliquot serum into a plastic vial and refrigerate. Protect specimen from light (place in brown plastic bag or aliquot into brown tube). Fractionated Bilirubin (BILIFRAC) instead of Total Bilirubin (TBIL) is recommended for neonates <14 days old. 

Special Preprations

1 No Special Preprations 


1 No Special Precautions 
 Test Name:- Bilirubin, total
  Specimen Type

 Test Name:- Bilirubin, total
 Test Name:- Bilirubin, total
 Test Name:-